Common name: Indian Wormwood, Fleabane, Mugwort • Hindi: नागदोना Nagdona, दवना Davana • Manipuri: লেইবাক ঙৌ Leibakngou. Natural products are the main stay of the alternative system of medicine. Artemisia nilagirica is commonly known as the Indian wormwood. Undershrubs, ca. m tall, aromatic, perennial, fruticose; stems paniculately branched, incanus, pubescent or tomentose. Leaves simple alternate, upper ones.
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A reliable protocol has been established for in vitro propagation of Artemisia nilagirica var. A highly proliferating organogenic callus was obtained on Murashige and Skoog MS medium supplemented with 2. Further, highest regeneration frequency An optimal of Ex-vitro plants were normal and were established successfully. Cytological and molecular marker studies showed that regenerated plants showed genetic stability in micro-propagated plants. It is also used as antihelmintic, antiseptic, antispasmodic, cholagogue, digestive, expectorant, purgative and stimulant.
The bioactive compounds isolated from A. The plant is also used traditionally in the preparation of insecticide Bhattacharjee Artemisia nilagirica is a promising species for extraction of an anti-malarial drug, artemisinin Shukla et al.
Habitat destruction for agricultural purposes and low seed viability is a major concern for the propagation of these plants.
Plant tissue culture techniques are useful for mass multiplication of threatened medicinal plants Rout et al. Consequently, the present artenisia is concerned to establish axenic cultures of medicinal plant Artemisia nilagirica var.
The pH of the media were adjusted to 5.
An organogenic callus from nodal explants obtained on MS medium supplemented with 2. To evaluate the growth potential of this callus for shoot induction, the callus was excised from the explants, divided into small pieces 0. The excised callus was transferred into MS medium containing 2.
One set was maintained as control without addition of any auxins. The plants were irrigated with Hoagland solution twice in a week. Randomised block design was used for setting up the experiments and each experiment was repeated 2 times with 6 replicates each. Percentage of responding explants, number of shoots per explant, shoot length, number of roots per shoot, root length, survival percentage in different potting mixture, plant height after hardening were observed and recorded.
Thirty regenerated plants from tissue culture were selected randomly and their qualitative and quantitative morphological traits aftemisia studied in detail. Morphological characters like vegetative and inflorescence were studied and recorded accordingly. The apices were then immersed in aceto-orceine: Isolation of genomic DNA from fresh leaves of mother and micropropagated plants of Artemisia nilagirica var.
The primers which were able to amplify the genomic DNA were nilagiricx for screening to analyze genetic variation between micro propagated plants with their mother plant. Amplification products were separated using 1. Callus was also induced from the nodal explants on medium supplemented with 2.
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The callogenetic response is also nilaigrica in various other species of ArtemisiaNin et al. In Artemisia pallensa semiorganized green callus with shoot buds on medium containing BAP and IAA and an unorganised callus on medium containing BAP and 2, 4-D from germinated seedlings has been reported by Benjamin et al. Similarly Nin et al. Effect on plant growth regulators on micropropagation of Artemisia nilagirica var.
The organogenic ability of the callus was observed by the application of 2. Medium supplemented with 2. Effect of different cytokinins on multiple shoot regeneration from callus cultures was previously reported in Jatropha curcas Maharana et al.
In Artemisia vulgaris it has nilatirica observed that shooting response was increased when BAP 4. Similar to the present results, callus mediated shoot regeneration was reported from seedling explants of Artemisia annua Fulzele et al.
Effect of cytokinins supplemented to MS medium on multiple shoot induction of Artemisia nilagirica var. Individual shoots were excised from the culture and inoculated on half and quarter strength medium with auxins viz.
In agreement with our finding, IBA was reported as a potential hormone for the induction of adventitious roots Arhemisia et al. Similar positive effect of lower artemksia strength of media nilagircia rhizogenesis has been reported earlier in Feronia limonia Hiregoudar et al. Effect of auxins supplemented to MS medium on root induction from the shoots of Artemisia nilagirica var. Successful transfer of in vitro grown plantlets to green house or field conditions is a crucial aspect in micropropagation Hazarika Plantlets with well developed roots were transferred to various potting mixtures for hardening in plastic cups.
The plantlets were initially maintained under controlled conditions Fig. The initial growth in plant height was 6. However the plant reached height up to nilagirlca. The frequency of ex-vitro survival and growth of acclimatized plant in different potting mixtures. The studied vegetative characters of the regenerated plants were similar with that of the mother plant.
Artemisia nilagirica () Pamp. | Species | India Biodiversity Portal
Both mother and in vitro plants flowered at the same time of the year during November to March. Similar stability in the morphology of in vitro grown Aloe vera was reported by Das et al. Cytological analysis revealed that there was no change in the chromosome number of micropropagated plants.
Similar reports showing the genetic stability of micropropagated plants was reported in Centaurea ultreiae and Nepenthes khasiana Mallon et nilagkrica. Clonal fidelity of in vitro regenerated Artemisia nilagirica var.
Primer UBC produced 5 monomorphic bands Fig. Thus, confirming the genetic stability of regenerants. Likewise, molecular markers were successfully used to reveal the genetic fidelity of micropropagated plants in Andrographis paniculata Dandin and Murthy bAtremisia absinthium Njlagirica et al.
Lane M DNA markers 1. Lanes 2—5ISSR profiles of four separate regenerated plantlets. In conclusion, a reliable in vitro plant regeneration method has been developed for the Artemisia nilagirica var. An optimal shoot regenerated from the nodal derived callus artfmisia MS medium supplemented 2. Morphological, cytological studies and ISSR marker analysis showed genetic stability of regenerants.
This protocol can be used for mass scale production of this important medicinal plant which could be used for plantation program and used as stock material for pharmaceutical uses. All the authors together written and approved nilagiric manuscript.
National Center for Biotechnology InformationU. Physiol Mol Biol Plants.
Published online Oct 3. Author information Article notes Copyright and License information Disclaimer. Hosakatte Niranjana Artmeisia, Email: Srivastava Foundation for Science and Society This article has been cited by other articles in PMC.
Abstract A reliable protocol has been established for in arte,isia propagation of Artemisia nilagirica var. Artemisia nilagiricaGenetic fidelity, Indian wormwood, Medicinal plant, Micropropagation, Multiple shoot regeneration. Introduction Artemisia nilagirica var. Materials and methods Plant material and explants establishment Nodal explants 1.
Multiple shoot regeneration from callus An organogenic callus from nodal explants obtained on MS medium supplemented with 2. Data collection and statistical analysis Randomised block design was used for setting up the experiments and each experiment was repeated 2 times with 6 replicates each.
Open in a separate window. Induction of multiple shoots from callus The organogenic ability of the callus was observed by argemisia application of 2.
Rooting of shoots Individual shoots were excised from the culture and inoculated on half and quarter strength medium with auxins viz. Acclimatization of rooted plantlets Successful transfer of in vitro grown plantlets to green house or field conditions is a crucial aspect in micropropagation Hazarika Genetic fidelity analysis Cytological analysis Cytological analysis revealed that there was no change in the chromosome number of micropropagated plants. Chromosome complements of Artemisia nilagirica var.
Compliance with ethical standards Conflict of interest Authors declare that they have no conflict of interest. Tissue culture of Artemisia pallens: Plant Cell Tissue Organ Cult. Hand book of medicinal plants. Enhanced in vitro multiplication of Nothapodytes nimmoniana Graham using semisolid and liquid cultures and estimation of camptothecin in the regenerated plants.
Artemisia (genus) – Wikipedia
nulagirica Regeneration of Andrographis paniculata Nees: Rapid nillagirica and analysis of genetic fidelity and scopoletin contents of micropropagated plants of Spilanthes oleracea L. J Hort Sci Biotechnol. Morphological and genetic characterization field grown plants of Aloe vera L.
Plant Tissue Cult Biotechnol. In vitro propagation of clonal fidelity of Nepenthes khasiana Hook. Roots as an enhancing factor for the production of artemisinin in shoot cultures of Artemisia annua. Tissue culture of Artemisia annua: Oil constituents of Artemisia nilagirica var. Acclimatization of tissue-cultured plants. In vitro cultgure of Feronia limonia L.